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Votre recherche pour: Protéines extraction

Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.

Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.


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Image du produit-PIER28340

Detergent sampler Kit, Surfact-Amps™

Numéro de catalogue: (PIER28340)
Fournisseur: Thermo Fisher Scientific
Description: Surfact-Amps™ Detergent sampler is a collection of various Surfact-Amps™ detergent solutions and selected other useful detergents for protein research methods.
UOM: 1 * 1 KIT
Image du produit-PIER20344

Pierce™, Detoxi-Gel™ Endotoxin Removing Columns and Gel

Fournisseur: Thermo Fisher Scientific
Description: Detoxi-Gel™ endotoxin removing columns and gel uses polymixin B ligand immobilised on beaded affinity resin to bind and extract endotoxins from antibody or protein samples.

Image du produit-PIER20230

Immobilised trypsin, TPCK treated, Pierce™

Numéro de catalogue: (PIER20230)
Fournisseur: Thermo Fisher Scientific
Description: Pierce™ immobilised TPCK trypsin is a serine endoprotease applicable to amino acid analysis and protein sequencing, mapping and structural studies. The immobilised form allows sample separation after treatment.
UOM: 1 * 2 mL
Image du produit-786-232

FOCUS™ Protein Alkylation Kit

Numéro de catalogue: (786-232)
Fournisseur: G-Biosciences
Description: The FOCUS™ Protein Alkylation Kit is a proteomic grade iodoacetamide reagent kit for blocking thiols by alkylation. The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimising reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups.
UOM: 1 * 1 KIT
Image du produit-786-792

Immobilised Trypsin

Numéro de catalogue: (786-792)
Fournisseur: G-Biosciences
Description: Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.
UOM: 1 * 1 ST
Image du produit-PIER90003

Phosphoprotein enrichment kit, Pierce™

Numéro de catalogue: (PIER90003)
Fournisseur: Thermo Fisher Scientific
Description: Pierce™ Phosphoprotein Enrichment Kit is designed for the efficient enrichment of phosphorylated proteins derived from mammalian cells and tissues.
UOM: 1 * 10 ST
Image du produit-PIER90058

Trypsin protease, MS grade

Fournisseur: Thermo Fisher Scientific
Description: Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.

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Image du produit-PIER89839

Organelle enrichment kits

Numéro de catalogue: (PIER89839)
Fournisseur: Thermo Fisher Scientific
Description: Organelle enrichment kit for lysosomes enables enrichment of intact organelles from cells and tissue. The kit provides the media and reagents necessary to accomplish subcellular fractionation using high-speed density gradient centrifugation.
UOM: 1 * 1 KIT
Image du produit-PIER89874

Mitochondria isolation kit for cultured cells

Numéro de catalogue: (PIER89874)
Fournisseur: Thermo Fisher Scientific
Description: The Mitochondria isolation Kit for cultured cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples in about 40 minutes.
UOM: 1 * 1 KIT
Image non disponible-71456-4

Master mix, BugBuster®

Fournisseur: Merck Millipore (Novagen)
Description: BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozyme™ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.

Image du produit-71009-4

Protein extraction reagents, CytoBuster™

Fournisseur: Merck Millipore (Novagen)
Description: The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.

Image non disponible-324715-5

Endoproteinase Lys-C, sequencing grade, Lysobacter enzymogenes

Numéro de catalogue: (324715-5)
Fournisseur: Merck Millipore (Calbiochem‎)
Description: Native endoproteinase Lys-C from <i>Lysobacter enzymogenes</i>. Serine protease that specifically hydrolyses amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease: protein by weight) for sequence analysis.
UOM: 1 * 5 µG
Image du produit-786-125

Protein concentration kit, OrgoSol-PROTEIN-Concentrate™

Numéro de catalogue: (786-125)
Fournisseur: G-Biosciences
Description: The OrgoSol-PROTEIN-Concentration™ Kit precipitates protein with a proprietary solvent buffer, OrgoSol™. The OrgoSol™ buffer has been specifically developed for efficient precipitation of protein solutions with minimal disruption to the protein structure and therefore maintains the biological activity of most proteins.
UOM: 1 * 50 mL
Image du produit-786-316

Cell surface protein isolation and labelling kit, HOOK™

Numéro de catalogue: (786-316)
Fournisseur: G-Biosciences
Description: The HOOK™ Cell Surface Protein Isolation Kit uses HOOK™ biotin labelling and purification technology in conjunction with Mammalian Cell PE LB™ Lysis Buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting. The Kit is supplied with all the necessary reagents and buffers for convenience and improved reproducibility. The kit is compatible with a wide variety of mammalian cells and can be used to compare treated and untreated cells and differences between different cell lines.
UOM: 1 * 1 KIT
Image non disponible-444810-1

Protein extraction kits, ProteoExtract™

Fournisseur: Merck Millipore (Calbiochem‎)
Description: ProteoExtract® Kits cover the different steps of the proteomics workflow, from protein extraction and abundant protein removal to concentration of protein mixtures and removal of interfering substances, digestion of proteins and selective capturing of phosphopeptides. These kits work in concert with one another to provide protein samples that are directly compatible with downstream analytical applications.

Image non disponible-324708-2

Endoproteinase Asp-N, excision grade, Pseudomonas fragi

Numéro de catalogue: (324708-2)
Fournisseur: Merck Millipore (Calbiochem‎)
Description: Native endoproteinase Asp-N from <i>Pseudomonas fragi.</i> Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyses proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
UOM: 1 * 2 µG
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