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Votre recherche pour: Protéines extraction

Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.

Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.


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Protein fractionation kit, FOCUS™ mitochondria

Numéro de catalogue: (786-022)
Fournisseur: G-Biosciences
Description: FOCUS™ Mitochondria kit is specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria. The majority (>90%) of the mitochondria have intact inner and outer membranes and therefore retain their functionality.
UOM: 1 * 1 KIT
Image du produit-786-258

Proteome kits, FOCUS™ Bacterial Proteome

Numéro de catalogue: (786-258)
Fournisseur: G-Biosciences
Description: Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilises nearly all of the proteins from <i>E.coli</i>, including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme enzyme, followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.
UOM: 1 * 1 KIT
Image du produit-786-251

Albumin removal kit, AlbuminOUT™

Fournisseur: G-Biosciences
Description: Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of albumin from such samples.

Image du produit-786-276

Antibody Fab and F(ab)₂ fragmentation of mouse IgG₁ kits

Fournisseur: G-Biosciences
Description: Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG<sub>1</sub> Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.

Image non disponible-ENZOADI9502730500

Antigen retrieval reagent, IHC

Fournisseur: ENZO LIFE SCIENCES
Description: Antigen retrieval reagent is used to recover antigens masked by fixation in cross linking fixatives such as formalin.

New Product

Image du produit-786-598

Biotin resin

Numéro de catalogue: (786-598)
Fournisseur: G-Biosciences
Description: Immobilised biotin resin is designed for the high affinity chromatography purifications of avidin, streptavidin and neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose.
UOM: 1 * 5 mL
Image du produit-786-249

Protein fractionation kit, FOCUS™ Membrane Proteins

Numéro de catalogue: (786-249)
Fournisseur: G-Biosciences
Description: FOCUS™ Membrane Proteins for the fractionation of highly enriched membrane proteins.
UOM: 1 * 1 KIT
Image du produit-786-257

FOCUS™ Yeast proteome kits

Numéro de catalogue: (786-257)
Fournisseur: G-Biosciences
Description: Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilises nearly all of the proteins from yeast, including membrane and soluble proteins. Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™ Zymolyase®, which has improved stability and shelf life. Enzymatic action is followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.
UOM: 1 * 1 KIT
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SilverOUT™, G-Biosciences

Numéro de catalogue: (786-244)
Fournisseur: G-Biosciences
Description: SilverOUT removes interfering silver ions from gel pieces.
UOM: 1 * 1 EA
Image du produit-786-793

Immobilised Ficin

Numéro de catalogue: (786-793)
Fournisseur: G-Biosciences
Description: Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave Immunoglobulin G (IgG) molecules in the hinge region.
UOM: 1 * 5 mL
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Optimizer blueBALLS™

Numéro de catalogue: (DGA01)
Fournisseur: G-Biosciences
Description: The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.
UOM: 1 * 500 ST
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InGel™ Blue for protein digestion

Numéro de catalogue: (786-681)
Fournisseur: G-Biosciences
Description: InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.
UOM: 1 * 1 KIT
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Bacterial Protein Extraction Kits, B-PER™

Fournisseur: Thermo Fisher Scientific
Description: B-PER® Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells. The B-PER® Bacterial Protein Extraction Kit eliminates the need for harsh mechanical procedures, like sonication. The easy-to-use cell lysis solution uses mild non-ionic detergents to disrupt cells and solubilise proteins without denaturation. Enzyme supplements such as DNase I and Lysozyme allow for improved yield of large molecular weight proteins which are difficult to purify. B-PER® is also compatible with downstream protein purification methods such as GST purification and His-tagged immobilised metal affinity chromatography (IMAC).

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EpiXtract® total histone extraction kit

Numéro de catalogue: (ENZOENZ450140100)
Fournisseur: ENZO LIFE SCIENCES
Description: Rapid, reliable method to isolate histone proteins in mammalian cells and tissues for histone modification applications.
UOM: 1 * 1 KIT

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Image non disponible-786-214

Detergent removal system, DetergentOUT™ Tween®

Fournisseur: G-Biosciences
Description: DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.

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Image du produit-786-247

Protein fractionation kit, FOCUS™ soluble and insoluble

Numéro de catalogue: (786-247)
Fournisseur: G-Biosciences
Description: FOCUS™ Soluble & Insoluble is a complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The kit comes with reagents necessary for fractionation of soluble and insoluble fractions, including a strong chaotropic extraction buffer to solubilise difficult proteins.
UOM: 1 * 1 KIT
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