Numéro de catalogue: (ROCKBCBT-100)

Fournisseur: Rockland Immunochemicals

Description: BCIP/TNBT Membrane Alkaline Phosphatase Substrate is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. BCIP/TNBT can be utilized in ELISA, in situ hybridization, and IHC. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of TNBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.

UOM: 1 * 100 mL

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Numéro de catalogue: (ROCKKAA064)

Fournisseur: Rockland Immunochemicals

Description: These assays are non-radioactive methods for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A specific dsDNA sequence containing the NF[kappa]B response element is immobilized on the bottom of the wells in a 96-well plate. The transcription factor is detected by the addition of a specific primary antibody. A colorimetric readout at 450 nm is obtained through a horseradish peroxidase-conjugated secondary antibody.

UOM: 1 * 1 KIT

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Numéro de catalogue: (1.00246.0250)

Fournisseur: Merck

Description: La solution de référence colorimétrique peut s'utiliser dans les comparaisons visuelles ou comme une partie de calibrage de colorimètres et de spectrophotomètres pour la comparaison des couleurs. La solution peut s'utiliser dans la préparation de solutions de référence avec différentes valeurs Hazen.

UOM: 1 * 250 mL

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Numéro de catalogue: (786-325)

Fournisseur: G-Biosciences

Description: ProteSEEKER™ identifies specific types of proteases with a panel of twelve protease inhibitors and a sensitive colorimetric protease screening assay. ProteSEEKER™ allows researchers to screen their protein samples and establish which specific class of proteases are present and therefore design a highly specific protease inhibitor cocktail using the minimal number of protease inhibitors. Alternatively, ProteSEEKER™ can be used to test existing protease inhibitor cocktails and identify their inadequacies and therefore supplement in additional protease inhibitors.

UOM: 1 * 50 Essai

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Fournisseur: Thermo Fisher Scientific

Description: Pierce™ streptavidin coated PS microplates are ideal for binding assays using biotinylated antibodies. High binding capacity (HBC) plates provide a wider detection range and better curve linearity for small biotinylated ligands, such as peptides and oligonucleotides, than the standard coated plates. Available in clear for colorimetric assays, white for chemiluminescent assays, and black for fluorescent assays.

Fournisseur: Thermo Fisher Scientific

Description: Pierce™ streptavidin coated PS microplates are ideal for binding assays using biotinylated antibodies. High binding capacity (HBC) plates provide a wider detection range and better curve linearity for small biotinylated ligands, such as peptides and oligonucleotides, than the standard coated plates. Available in clear for colorimetric assays, white for chemiluminescent assays, and black for fluorescent assays.

Fournisseur: MP Biomedicals

Description: p-Nitroblue Tetrazolium Chloride is an NADPH-diaphorase substrate that competitively inhibits NOS (nitric oxide synthase) (IC50 = 3-4 μM). In combination with 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and an appropriate reaction buffer, Nitroblue tetrazolium forms a substrate for the colorimetric detection of alkaline phosphatase conjugates in a variety of molecular and cell biology techniques.

p-nitro blue tetrazolium is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.

Fournisseur: MP Biomedicals

Description: Storage: +4°C, protect from light
3- (4,5-Dimethyl-2-thiazolyl-2)-2,5-diphenyl-tetrazolium bromide is used as a colorimetric metabolic activity indicator in cell viability assays. Thiazolyl Blue Tetrazolium Blue (MTT) is a dye used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

MSMD

Fournisseur: Cytiva

Description: Amersham™ Protran® membranes are manufactured from 100% pure nitrocellulose to ensure the highest binding capacity possible. They are compatible with a variety of detection methods, including isotopic, chemiluminescent, colorimetric and fluorescent. No methanol pre-wetting step is required, making this the membrane of choice for proteins which prefer aqueous environments. Prior to transfer the membrane is simply wetted with water, and then placed in the transfer buffer. No other pretreatment steps are necessary.

Fournisseur: Whatman products (Cytiva)

Description: Protran® membranes are manufactured from 100% pure nitrocellulose to ensure the highest binding capacity possible. They are compatible with a variety of detection methods, including isotopic, chemiluminescent, colorimetric and fluorescent. No methanol pre-wetting step is required, making this the membrane of choice for proteins which prefer aqueous environments. Prior to transfer the membrane is simply wetted with water, and then placed in the transfer buffer. No other pre treatment steps are necessary.

Fournisseur: Thermo Fisher Scientific

Description: These Ni(2+) chelate-coated plates are ideal for analysing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking. The clear, white or black plates can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.

Fournisseur: SIGMA ALDRICH MICROSCOPY

Description: Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

Fournisseur: Thermo Fisher Scientific

Description: Thermo Scientific Pierce coomassie brilliant blue dyes are composed of one of the most common forms of coomassie dye, which is a key component of various colorimetric protein gel stains. Coomassie R-250 and G-250 dyes are two chemical forms of a disulphonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis and bradford-type assay reagents for protein quantitation. The R-250 (red-tinted) form lacks two methyl groups that are present in the G-250 (green-tinted) form, which is also called colloidal coomassie dye.

Certificats

Numéro de catalogue: (HANNHI98494)

Fournisseur: Hanna

Description: MULTIPARAMÈTRE PORTATIF PH/RÉDOX/EC/LDO 1 * 1 Pce

UOM: 1 * 1 ST

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Numéro de catalogue: (368057-5)

Fournisseur: Merck Millipore (Calbiochem‎)

Description: GRANZYME B SUBSTRATE I, COLORIMETRIC 1 * 5 mg

UOM: 1 * 5 mg

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Numéro de catalogue: (GENO786-1807)

Fournisseur: G-Biosciences

Description: PROTEINASE K ASSAY [COLORIMETRIC] 1 * 1 KIT

UOM: 1 * 1 KIT

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