Votre recherche pour: Merck Millipore (Novagen)

Fournisseur: Merck Millipore (Novagen)

Description: A common method for transformation of plasmids into <i>E.coli</i> is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Fournisseur: Merck Millipore (Novagen)

Description: Biotinylated Thrombin has covalently attached biotin for easy removal of the enzyme from cleavage reactions using immobilised streptavidin. This preparation is tested for activity using the same assay as for unmodified thrombin, and for >99% binding to streptavidin agarose.

Fournisseur: Merck Millipore (Novagen)

Description: BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozyme™ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.

Fournisseur: Merck Millipore (Novagen)

Description: Novagen® T7Select® Phage Display System takes advantage of the properties of bacteriophage T7. This system is easy to use and has the capacity to display peptides up to about 50 amino acids in size in high copy number (415 per phage), and peptides or proteins up to about 1200 amino acids in low copy number (0,1 to 1 per phage) or mid-copy number (5 to 15 per phage).

Numéro de catalogue: (71366-4)

Fournisseur: Merck Millipore (Novagen)

Description: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

UOM: 1 * 1 ST

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Numéro de catalogue: (71610-3)

Fournisseur: Merck Millipore (Novagen)

Description: D-Desthiobiotin provides a gentle elution of StrepTag® II proteins from the biotin-binding site of StrepTactin® resins. D-Desthiobiotin is offered as a lyophilised powder for preparation of elution buffer.

UOM: 1 * 1 g

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Fournisseur: Merck Millipore (Novagen)

Description: BugBuster® 10X est une formulation concentrée des détergents exclusifs utilisés dans BugBuster® sans ajout de sels ou de composants tampon. Le réactif BugBuster® concentré offre une alternative flexible au réactif BugBuster® 1X standard prêt à l'emploi, permettant ainsi une dilution personnalisée et l'ajout de composants tampon. BugBuster® 10X présente tous les avantages du réactif BugBuster® standard en matière de biotraitement, mais il offre également la liberté de contrôler le pH, la concentration en réactif et les ajouts de tampon nécessaires pour une activité et une extraction maximales de vos protéines cibles.

Fournisseur: Merck Millipore (Novagen)

Description: Le réactif de transfection Insect GeneJuice® est formulé pour optimiser l'efficacité de la transfection des cellules d'insectes Sf9. Le réactif apporte une toxicité extrêmement faible aux cellules et peut être utilisé pour les transfections transitoires et stables dans les milieux avec ou sans sérum, et pour la cotransfection des plasmides de transfert avec ADN de virus linéarisé pour la production de baculovirus recombinés.

Fournisseur: Merck Millipore (Novagen)

Description: THP [Tris(hydroxypropyl)phosphine; C9H21O3P] is a water-soluble, odorless, sulfhydryl reducing agent that is more stable and effective than dithiothreitol (DTT).

Fournisseur: Merck Millipore (Novagen)

Description: Lysonase™ bioprocessing reagent is an optimised, ready-to-use convenient blend of rLysozyme™ solution and benzonase nuclease which is ideal for protein extraction.

MSMD

Fournisseur: Merck Millipore (Novagen)

Description: The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.

Numéro de catalogue: (69959-3)

Fournisseur: Merck Millipore (Novagen)

Description: The Perfect Protein™ Western Markers consist of seven recombinant S•Tag™/His•Tag® fusion proteins that can be detected on any Western blot using an S-protein AP or HRP conjugate. As an additional option, the markers can also be detected with an His•Tag Monoclonal Antibody and an AP or HRP conjugated secondary antibody. The Perfect Protein Western Markers serve as precise size standards that appear simultaneously on the blot with target proteins, eliminating the uncertainty and imprecision associated with other methods.

UOM: 1 * 1 ST

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Fournisseur: Merck Millipore (Novagen)

Description: The pSTBlue-1 AccepTor™ vector kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (eg. KOD XL polymerase and native and recombinant <i>Taq</i> polymerases), which leave single 3'-dA overhangs on the reaction products. The AccepTor™ vectors enable direct ligation by virtue of single 3'-dU overhangs that anneal efficiently with 3'-dA extensions on PCR products. The dU residues are converted to dT residues<i> in vivo</i> following transformation.

Fournisseur: Merck Millipore (Novagen)

Description: Les dialyseurs D-tube96™ permettent une dialyse simultanée pratique et à haut rendement de 96 échantillons. Le dispositif offre les avantages du dialyseur Mini D-Tube™ dans un format de 96 tubes. Les dialyseurs D-Tube sont des tubes faciles à manipuler comportant une double membrane fournissant une grande surface pour des dialyses rapides et efficaces. La membrane est ultra-propre, traitée à l'EDTA, en cellulose régénérée exempte de soufre et de métaux lourds.

Numéro de catalogue: (70527-3)

Fournisseur: Merck Millipore (Novagen)

Description: The lac operon inducer IPTG (isopropyl-β-D- 1-thiogalactopyranoside) is recommended for blue/white screening by lacZ α- complementation with appropriate vectors and host strains, such as pSTBlue-1/NovaBlue, and for inducing protein expression with the pET System and other lac promoter-derived expression systems.

UOM: 1 * 15 mL

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Numéro de catalogue: (69755-3)

Fournisseur: Merck Millipore (Novagen)

Description: The His·Bind buffer kit is a set of pre-tested buffers designed for use with IDA-based His·Bind resin for convenient, rapid one-step purification of proteins by metal chelation chromatography.

UOM: 1 * 1 KIT

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