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Fournisseur: FINNZYMES REAGENTS

Description: L'ADN polymérase Phusion® génère des produits de la PCR avec une exactitude et une rapidité inaccessibles avec une seule enzyme, même sur les modèles les plus difficiles. La structure et les caractéristiques de l'ADN polymérase Phusion® haute fidélité en font le meilleur choix pour le clonage et redéfinissent les normes en matière de performance de PCR avec un taux d'erreurs 50 fois plus faible que pour l'ADN polymérase <i>Taq</i> et 6 fois plus faible que pour l'ADN polymérase <i>Pfu</i>. En outre, l'ADN polymérase Phusion® haute fidélité présente une processivité 10 fois supérieure à celle de l'ADN polymérase <i>Pfu</i>.

MSMD

Fournisseur: FINNZYMES REAGENTS

Description: Phusion® High-Fidelity PCR Master Mix with HF Buffer is a convenient 2X mix containing Phusion® DNA Polymerase, nucleotides and optimised HF buffer including MgCl₂. Only template and primers need to be added by the user.

Fournisseur: FINNZYMES REAGENTS

Description: Phusion® U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Fournisseur: FINNZYMES REAGENTS

Description: Phire™ Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects. The master mix containing Phire™ Hot Start II DNA polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors, such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle).

Fournisseur: FINNZYMES REAGENTS

Description: Phusion® High-Fidelity PCR Master Mix with GC Buffer is a convenient 2X mix containing Phusion® DNA polymerase, nucleotides and optimised GC buffer including MgCl₂. Only template and primers need to be added by the user.

Fournisseur: FINNZYMES REAGENTS

Description: Phusion® U multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

Fournisseur: FINNZYMES REAGENTS

Description: Phusion® high-fidelity DNA polymerases set a gold standard for high performance PCR.

Fournisseur: FINNZYMES REAGENTS

Description: Phire® Hot Start II DNA Polymerase is faster, extremely robust, and capable of amplifying long DNA fragments with high yields. Phire® Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to <i>Taq</i> DNA polymerase. In addition, the Hot Start technology allows complete reactivation of the enzyme in “zero-time” at standard cycling temperatures. This combination of features makes the polymerase an ideal solution for routine and high throughput PCR applications. Phire® Hot Start II DNA Polymerase delivers superior performance in conventional thermal cyclers as well as in fast instruments, such as the Piko® Thermal Cycler.

Fournisseur: FINNZYMES REAGENTS

Description: Phusion® Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion® Hot Start II High-Fidelity DNA Polymerase is resistant to main PCR inhibitors present in blood and retains its activity at blood concentrations of up to 40% in the PCR reaction.

Fournisseur: FINNZYMES REAGENTS

Description: Phusion® U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Fournisseur: FINNZYMES REAGENTS

Description: L'ADN polymérase Phusion® Hot Start II haute fidélité permet l'utilisation d'un large éventail d'amorces, y compris celles avec de faibles températures de fusion. L'ADN polymérase Phusion® Hot Start haute fidélité est idéal pour les applications de PCR, des PCR de routine aux applications très exigeantes, telles que le clonage et la PCR à haut rendement. Il est équipé d'un domaine à liaison d'ADN bicaténaire unique, ce qui rend cette ADN polymérase avec une activité de correction de type Pyrococcus extrêmement robuste, rapide et précise.

Fournisseur: FINNZYMES REAGENTS

Description: Phusion® Green Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion® Hot Start II DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® Hot Start II DNA polymerase and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Fournisseur: FINNZYMES REAGENTS

Description: DyNAmo™ qPCR Kits offer excellent performance in detection and quantitation of DNA and RNA sequences from various sources. The DyNAmo™ qPCR kit family features optimised kits for fast and conventional qPCR. Kits utilising either SYBR® Green or probe chemistries for various platforms are available. All DyNAmo™ qPCR kits are provided as convenient 2X master mixes - only template and primers need to be added.

Fournisseur: FINNZYMES REAGENTS

Description: Phusion® Green High-Fidelity DNA Polymerase is a combination of Phusion® DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® DNA Polymerases and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Fournisseur: FINNZYMES REAGENTS

Description: Direct PCR Kits save time and cost by allowing amplification of DNA directly from the source material. Direct PCR is based on Thermo Scientific Phire® Hot Start II DNA Polymerase and Phusion® Hot Start II High-Fidelity DNA Polymerase. These fusion enzymes are exceptionally fast, robust and highly tolerant of many PCR inhibitors present in unpurified sample material. This allows reliable amplification in challenging conditions where conventional DNA polymerases are completely inhibited.

Fournisseur: FINNZYMES REAGENTS

Description: Phire® Hot Start II DNA Polymerase is faster, extremely robust, and capable of amplifying long DNA fragments with high yields. Phire® Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to <i>Taq</i> DNA polymerase. In addition, the Hot Start technology allows complete reactivation of the enzyme in “zero-time” at standard cycling temperatures. This combination of features makes the polymerase an ideal solution for routine and high throughput PCR applications. Phire® Hot Start II DNA Polymerase delivers superior performance in conventional thermal cyclers as well as in fast instruments, such as the Piko® Thermal Cycler.