Votre recherche pour: Thermo Fisher Scientific

Fournisseur: Thermo Fisher Scientific

Description: Catalyses the transfer of the γ-phosphate from ATP to the 5'-hydroxyl group of single- and double-stranded DNAs and RNAs, oligonucleotides, or nucleoside 3'-monophosphates (forward reaction).

Numéro de catalogue: (EN0525)

Fournisseur: Thermo Fisher Scientific

Description: DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyses phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'- phosphate and 3'- OH groups.

UOM: 1 * 1 KIT

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Fournisseur: Thermo Fisher Scientific

Description: RevertAid™ H Minus Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to point mutation in the RNase H domain. RevertAid™ H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA and therefore high yields of full-length cDNA from long templates are obtained. RevertAid™ H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 55 °C), which makes the enzyme an ideal tool for reverse transcription of RNAs having a high degree of secondary structure.

Fournisseur: Thermo Fisher Scientific

Description: GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer.

Numéro de catalogue: (R0861)

Fournisseur: Thermo Fisher Scientific

Description: Dithiothreitol (DTT, Réactif de Cleland)

UOM: 1 * 5 g

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Numéro de catalogue: (ER1751)

Fournisseur: Thermo Fisher Scientific

Description: CCNGGA sites and cuts best at 37 °C in Tango buffer.

UOM: 1 * 1 KIT

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Fournisseur: Thermo Fisher Scientific

Description: Maxima™ Reverse Transcriptase is an advanced enzyme derived by <i>in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR. The Maxima™ Reverse Transcriptase is capable of cDNA synthesis at elevated temperatures (50 to 60 °C) and completion of the reverse transcription reaction in 15 to 30 minutes. The use of this enzyme in combination with Maxima™ qPCR Master Mixes ensures high specificity, sensitivity and wide linear range of two step RT-qPCR. Applications include first strand cDNA synthesis, RT-PCR, and RT-qPCR.

Numéro de catalogue: (FD1464)

Fournisseur: Thermo Fisher Scientific

Description: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

UOM: 1 * 1 KIT

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Fournisseur: Thermo Fisher Scientific

Description: The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host <i>E. coli</i> cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

Numéro de catalogue: (ER1921)

Fournisseur: Thermo Fisher Scientific

Description: CCWGG sites and cuts best at 37 °C in O buffer.

UOM: 1 * 1 KIT

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Numéro de catalogue: (EO0461)

Fournisseur: Thermo Fisher Scientific

Description: Agarase is an enzyme that specifically digests the agarose polysaccharide core into neoagaro-oligosaccharides.

UOM: 1 * 1 KIT

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Numéro de catalogue: (ER0303)

Fournisseur: Thermo Fisher Scientific

Description: ATC sites and cuts best at 37 °C in R buffer Isoschizomers: EcoRV.

UOM: 1 * 1 KIT

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Fournisseur: Thermo Fisher Scientific

Description: Thermo Scientific 5-Fluoroorotic Acid (5-fluorouracil-6-carboxylic acid monohydrate; 5-FOA) is used in yeast molecular genetics to detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase

Numéro de catalogue: (R0862)

Fournisseur: Thermo Fisher Scientific

Description: Dithiothreitol (DTT, Réactif de Cleland)

UOM: 1 * 1 KIT

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Fournisseur: Thermo Fisher Scientific

Description: Exonuclease I (ExoI) degrades single-stranded DNA in a 3' to 5'direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.

Numéro de catalogue: (ER0053)

Fournisseur: Thermo Fisher Scientific

Description: GATCC sites and cuts best at 37 °C in its own unique buffer.

UOM: 1 * 1 KIT

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