Votre recherche pour: Thermo Fisher Scientific

Fournisseur: Thermo Fisher Scientific

Description: GeneJET™ Plasmid Midiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant <i>E. coli</i> cultures. The kit utilises silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 µg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing,<i> in vitro</i> transcription, and transfection of robust cell lines.

Numéro de catalogue: (K1612)

Fournisseur: Thermo Fisher Scientific

Description: The First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses M-MuLV Reverse Transcriptase, which has lower RNase H activity compared to AMW reverse transcriptase. The enzyme maintains activity at 37 °C and is suitable for synthesis of cDNA up to 9 kb. The recombinant Thermo Scientific RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55 °C.

UOM: 1 * 1 KIT

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Numéro de catalogue: (SO142)

Fournisseur: Thermo Fisher Scientific

Description: Random hexamer primer is a mixture of single-stranded random hexanucleotides with 5'- and 3'-hydroxyl ends. The primer is supplied as a ready to use, 20X concentrated aqueous solution. Random hexamer primer has been functionally tested in first strand cDNA synthesis.

UOM: 1 * 1 KIT

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Numéro de catalogue: (EN0771)

Fournisseur: Thermo Fisher Scientific

Description: Thermo Scientific dsDNase is an engineered shrimp DNase designed for rapid and safe removal of contaminating genomic DNA from RNA samples. It is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. Highly specific activity towards double-stranded DNA ensures that RNA and single-stranded DNA, such as cDNA and primers are not cleaved. dsDNase is easily inactivated by moderate heat treatment (55 °C). These features make dsDNase an excellent choice for gDNA elimination prior reverse transcription. It allows for dramatically simplified workflow which combines genomic DNA elimination and cDNA synthesis into one-tube procedure.

UOM: 1 * 1 ST

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Fournisseur: Thermo Fisher Scientific

Description: Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that ensures higher PCR specificity, sensitivity and yields compared to conventional hot start Taq DNA polymerases. DreamTaq™ Hot Start DNA Polymerase uses antibody based inhibition of DNA polymerase activity at ambient temperatures, thus preventing the amplification of non-specific products prior to the amplification step.

Fournisseur: Thermo Fisher Scientific

Description: The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host <i>E. coli</i> cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

Numéro de catalogue: (FD1464)

Fournisseur: Thermo Fisher Scientific

Description: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

UOM: 1 * 1 KIT

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Numéro de catalogue: (ER1921)

Fournisseur: Thermo Fisher Scientific

Description: CCWGG sites and cuts best at 37 °C in O buffer.

UOM: 1 * 1 KIT

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Fournisseur: Thermo Fisher Scientific

Description: Exonuclease I (ExoI) degrades single-stranded DNA in a 3' to 5'direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.

Numéro de catalogue: (EO0461)

Fournisseur: Thermo Fisher Scientific

Description: Agarase is an enzyme that specifically digests the agarose polysaccharide core into neoagaro-oligosaccharides.

UOM: 1 * 1 KIT

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Numéro de catalogue: (R0862)

Fournisseur: Thermo Fisher Scientific

Description: Dithiothreitol (DTT, Réactif de Cleland)

UOM: 1 * 1 KIT

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Fournisseur: Thermo Fisher Scientific

Description: Thermo Scientific 5-Fluoroorotic Acid (5-fluorouracil-6-carboxylic acid monohydrate; 5-FOA) is used in yeast molecular genetics to detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase

Numéro de catalogue: (ER0303)

Fournisseur: Thermo Fisher Scientific

Description: ATC sites and cuts best at 37 °C in R buffer Isoschizomers: EcoRV.

UOM: 1 * 1 KIT

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Numéro de catalogue: (ER0053)

Fournisseur: Thermo Fisher Scientific

Description: GATCC sites and cuts best at 37 °C in its own unique buffer.

UOM: 1 * 1 KIT

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Numéro de catalogue: (FD1014)

Fournisseur: Thermo Fisher Scientific

Description: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

UOM: 1 * 1 KIT

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Fournisseur: Thermo Fisher Scientific

Description: Luminaris Color Probe qPCR Master Mixes are universal ready-to-use solutions optimised for qPCR and two-step RT-qPCR. The master mixes include hot start <i>Taq</i> DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimised PCR buffer. Only the template, primers and probe need to be added.