Votre recherche pour: Merck+Millipore+(Novagen)

Fournisseur: Merck Millipore (Novagen)

Description: BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

Fournisseur: Merck Millipore (Novagen)

Description: pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand.

Fournisseur: Merck Millipore (Novagen)

Description: A common method for transformation of plasmids into <i>E.coli</i> is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Fournisseur: Merck Millipore (Novagen)

Description: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

Numéro de catalogue: (72181-3)

Fournisseur: Merck Millipore (Novagen)

Description: 293-Free™ transfection reagent has a unique polycationic liposomal formulation and is designed expressly for transfection of HEK 293 cells grown in suspension culture. It is ideal for mammalian protein production. 293-Free™ transfection reagent is provided as a sterile, ready to use solution. The 1 ml size provides enough reagent to transfect a litre of culture.

UOM: 1 * 1 mL

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Numéro de catalogue: (71285-3)

Fournisseur: Merck Millipore (Novagen)

Description: BCA Protein Assay is a simple and reliable protein quantification method. It can be used to determine protein concentration in the range of 20-2000 µg/ml in either a standard assay or microassay configuration.

UOM: 1 * 1 KIT

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Fournisseur: Merck Millipore (Novagen)

Description: Le réactif d'extraction de protéines BugBuster® est formulé de façon à rompre délicatement la paroi cellulaire de l'E. coli, afin de libérer la protéine soluble. Il offre une alternative simple, rapide et économique aux méthodes mécaniques (French Press ou sonication) d'extraction de protéines cibles à des fins de purification ou d'autres applications. La formulation utilise un mélange de détergents capable de perforer la paroi cellulaire sans dénaturer la protéine soluble.

Fournisseur: Merck Millipore (Novagen)

Description: Biotinylated Thrombin has covalently attached biotin for easy removal of the enzyme from cleavage reactions using immobilised streptavidin. This preparation is tested for activity using the same assay as for unmodified thrombin, and for >99% binding to streptavidin agarose.

Fournisseur: Merck Millipore (Novagen)

Description: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

Numéro de catalogue: (69704-4)

Fournisseur: Merck Millipore (Novagen)

Description: S-protein agarose specifically retains STag™ fusion proteins. The capacity of S-protein agarose varies and is based on the size and folding characteristics of a given target protein.

UOM: 1 * 10 mL

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Numéro de catalogue: (71610-3)

Fournisseur: Merck Millipore (Novagen)

Description: D-Desthiobiotin provides a gentle elution of StrepTag® II proteins from the biotin-binding site of StrepTactin® resins. D-Desthiobiotin is offered as a lyophilised powder for preparation of elution buffer.

UOM: 1 * 1 g

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Fournisseur: Merck Millipore (Novagen)

Description: Benzonase® nuclease degrades all forms of DNA and RNA while having no proteolytic activity. Benzonase® is compatible with BugBuster® and PopCulture® protein extraction reagents and therefore can be added to these reagents to eliminate viscosity and remove nucleic acids from <i>E. coli </i>extracts.

Fournisseur: Merck Millipore (Novagen)

Description: This His Tag® monoclonal antibody is a mouse monoclonal antibody (IgG1) directed against the His tag sequence encoded by many of the expression vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context.

Numéro de catalogue: (71366-4)

Fournisseur: Merck Millipore (Novagen)

Description: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

UOM: 1 * 1 ST

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Fournisseur: Merck Millipore (Novagen)

Description: Les échelles Perfect DNA™ contiennent des jeux d'espèces d'DNA de taille pratique et aisément mémorisable pour l'analyse de gel d'agarose. Les marqueurs ont des intensités de bande uniformes et couvrent une large gamme de tailles d'DNA.

Fournisseur: Merck Millipore (Novagen)

Description: BugBuster® 10X est une formulation concentrée des détergents exclusifs utilisés dans BugBuster® sans ajout de sels ou de composants tampon. Le réactif BugBuster® concentré offre une alternative flexible au réactif BugBuster® 1X standard prêt à l'emploi, permettant ainsi une dilution personnalisée et l'ajout de composants tampon. BugBuster® 10X présente tous les avantages du réactif BugBuster® standard en matière de biotraitement, mais il offre également la liberté de contrôler le pH, la concentration en réactif et les ajouts de tampon nécessaires pour une activité et une extraction maximales de vos protéines cibles.