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Master mixes, multiplex, Phusion® U

Fournisseur: FINNZYMES REAGENTS
Description: Phusion® U multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

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Master mixes, green multiplex, Phusion® U

Fournisseur: FINNZYMES REAGENTS
Description: Phusion® U green multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

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Billes PCR, illustra™ PuReTaq Ready-To-Go™

Fournisseur: Cytiva
Description: PuReTaq RTG PCR beads and reagents ensures reliable and robust performance in both end point and RT-PCR amplifications and ensures the lowest possible levels of contaminating prokaryotic and eukaryotic nucleic acids.

Image non disponible-ENZOADIEKS390A

PKA kinase activity kit

Numéro de catalogue: (ENZOADIEKS390A)
Fournisseur: ENZO LIFE SCIENCES
Description: The PKA Kinase activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.
UOM: 1 * 1 KIT
Image non disponible-ENZOADIEKS420A

PKC kinase activity kit

Numéro de catalogue: (ENZOADIEKS420A)
Fournisseur: ENZO LIFE SCIENCES
Description: The PKC Kinase activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.
UOM: 1 * 1 KIT
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PCR clean-up kit, Axygen® AxyPrep™ Mag

Fournisseur: Corning
Description: The efficient removal of unincorporated dNTPs, salts and enzymes after any PCR is detrimental to the success or failure of downstream applications, such as genotyping, Sanger, and Next Generation Sequencing. The AxyPrep™ Mag PCR Clean Up Kit utilises a paramagnetic bead technology for rapid high throughput purification of PCR products. Additionally, the AxyPrep™ Mag PCR Clean Up Kit employs an optimised buffer to selectively bind PCR amplicons 60 bp and larger to paramagnetic beads, providing end users flexibility and high recovery options. The protocol comprises binding, washing and eluting steps, where primers, nucleotides, salts and enzymes in the reaction mixture are efficiently removed leaving a purified PCR product that is essentially free of contaminants. No centrifugation or filtration steps are required, allowing the process to be adapted to multiple commercially available automation platforms.

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Taq DNA polymerase, MP Biomedicals

Fournisseur: MP Biomedicals
Description: <i>Taq</i> DNA Polymerase is a specialised thermostable enzyme isolated from the thermophilic bacterium <i>Thermus aquaticus</i>. The recombinant form of this enzyme is expressed in <i>E. coli</i>. It is highly processive, recombinant polymerase which catalyses the addition of mononucleotide units to the 3’-OH end of a primer chain. It remains functional even after prolonged incubation at 95 °C. This enzyme possesses 5’-3’ exonuclease activiy.

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Taq DNA polymerase (recombinant)

Fournisseur: Thermo Fisher Scientific
Description: <i>Taq</i> DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium <i>Thermus aquaticus</i>. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, <i>Taq</i> DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant <i>Taq</i> DNA Polymerase is ideal for standard PCR of templates 5 kb or shorter.

Certificats

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Taq DNA polymerase (native, without BSA)

Numéro de catalogue: (EP0282)
Fournisseur: Thermo Fisher Scientific
Description: <i>Taq</i> DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium <i>Thermus aquaticus</i>. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, <i>Taq</i> DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3’-end of PCR products. Native <i>Taq</i> DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in <i>E.coli</i>.
UOM: 1 * 1 KIT
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Taq DNA polymerase (native, with BSA)

Numéro de catalogue: (EP0072)
Fournisseur: Thermo Fisher Scientific
Description: <i>Taq</i> DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium <i>Thermus aquaticus</i>. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, <i>Taq</i> DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native <i>Taq</i> DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in <i>E.coli</i>. Native<i> Taq</i> DNA polymerase stabilised with BSA is often the best choice when amplifying DNA samples of lower purity, e.g. genomic DNA from mouse tail.
UOM: 1 * 1 KIT
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Pfu DNA polymerase

Fournisseur: G-Biosciences
Description: <i>Pfu</i> DNA Polymerase, derived from the hyperthermophilic archae <i>Pyrococcus furiosus</i>, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). <i>Pfu</i> DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that<i> Pfu</i> DNA Polymerase-generated PCR fragments will have fewer errors than <i>Taq</i>-generated PCR inserts. Using <i>Pfu</i> DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. <i>Pfu</i> DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

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Peqlab, Components for Peqlab DNA Polymerases

Numéro de catalogue: (PEQL01-1018)
Fournisseur: VWR Peqlab
Description: 10X PCR Buffer, incomplete
UOM: 1 * 500 mL

Certificats


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ADN polymérase Taq sans glycérol, VWR®

Fournisseur: VWR Chemicals
Description: L'ADN polymérase Taq hautement concentrée, sans glycérol, 50 U/µl, est idéale pour l'utilisation industrielle et la fabrication de kits de diagnostic PCR.

MSMD

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Restriction enzymes, rTaqPDNA

Fournisseur: Apollo Scientific
Description: Recombinant Taq plus DNA polymerase produced in <i>E.coli </i>5 U/µl.

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VWR® Fast extract genotyping PCR kit

Fournisseur: VWR Chemicals
Description: This fast extract genotyping PCR kit consists of a fast extraction DNA solution and VWR® Red Taq DNA polymerase 2X master mix. It is ideal for genotyping of DNA extracted from mammalian tissues, plant material, saliva or bacteria, providing PCR-ready DNA in 8 minutes and genotyping results in less than 1,5 hours.

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Genomic DNA extraction from dried blood spots, Extracta™ DBS

Fournisseur: Quantabio
Description: Extracta™ DBS is a ready to use DNA extraction reagent for rapid and efficient recovery of PCR-ready DNA from dried blood spots (DBS) (https://fr.vwr.com/store/product/28387399/biosample-cards) on Guthrie cards or Whatman® 903 filter/TFN or 226 from Ahlstrom Munksjö paper. This patented single-solution process produces DNA eluates that are substantially free of PCR inhibitors and compatible with a variety of end-point PCR, real-time PCR and Next Generation Sequencing (NGS) or Sanger Sequencing reagents. Application of Extracta DBS with PerfeCTa® qPCR ToughMix® or PerfeCTa MultiPlex qPCR ToughMix® enables accurate and reproducible quantification of DNA sequences in blood using TaqMan® hydrolysis probe real-time qPCR.

MSMD

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