Description: SuperSignal™ West HisProbe Kit contains HisProbe-HRP conjugate and reagents required for detection of polyhistidine-tagged protein by Western blotting. HisProbe-HRP Conjugate is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins.

Numéro de catalogue: PIER15168

UOM: 1 * 1 KIT

Fournisseur: Thermo Fisher Scientific

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Description: The Pierce™ Metal enhanced DAB substrate kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate that yields intense colour for immunohistochemical staining.

Numéro de catalogue: PIER34065

UOM: 1 * 250 mL

Fournisseur: Thermo Fisher Scientific

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Description: Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis. The presence of contaminants, or interfering agents, such as salts, acids, bases and detergents, result in band distortion and poor protein resolution. As a result, SDS-PAGE gels are hard to analyse and lack reproducibility. PAGE-Perfect™ is a simple, two-step method for concentrating, cleaning and preparing protein solutions for running publication quality gels.

Numéro de catalogue: 786-123

UOM: 1 * 1 KIT

Fournisseur: G-Biosciences

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Description: The Perfect Protein™ HRP Western Blot Kit includes an HRP or AP conjugate in addition to Perfect Protein™ Western markers.

Numéro de catalogue: 69078-3

UOM: 1 * 1 ST

Fournisseur: Merck Millipore (Novagen)

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Description: FemtoMax™ is an extremely sensitive, non radioactive, and enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP). It allows for the detection of femtogram (10 to 15) amounts of antigen using photographic film or other imaging methods including highly sensitive CCD cameras.

Numéro de catalogue: ROCKFEMTOMAX-020

UOM: 1 * 20 mL

Fournisseur: Rockland Immunochemicals

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Description: SSC solution is commonly used in nucleic acid hybridization techniques at concentrations ranging from 0,1X to 20X depending on the application. It is used to facilitate transfer of nucleic acids to membranes in Southern and Northern blotting, dot blotting, and colony and plaque lifts.

Numéro de catalogue: J60561.AK

UOM: 1 * 250 mL

Fournisseur: Thermo Fisher Scientific

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Description: The Fast Western Blot Kit, SuperSignal™ West Pico Substrate contains optimised reagents and protocol for picogram-level detection and and Western blot development less than 1 hour after gel transfer.

Numéro de catalogue: PIER35061

UOM: 1 * 1 KIT

Fournisseur: Thermo Fisher Scientific

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Description: TrueBlot® Western blot kits contain the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples using TrueBlot® second step immunoblotting reagents in conjunction with the user's own primary IP antibody and primary (mouse, goat or rabbit IgG) Western blotting antibody.

Numéro de catalogue: ROCK18-8815-31

UOM: 1 * 50 µl

Fournisseur: Rockland Immunochemicals

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Description: This Western-Re-Probe™ reagent is validated for use in immunoblotting.

Numéro de catalogue: WB59-100

UOM: 1 * 100 mL

Fournisseur: Merck Millipore (Oncogene)

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Description: The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes.

Numéro de catalogue: PIER35081

UOM: 1 * 1 KIT

Fournisseur: Thermo Fisher Scientific

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Description: Perfect-FOCUS™ is based on quantitative precipitation and concentration of protein solutions using UPPA™ (Universal Protein Precipitation Agent) and has been specifically developed for preparing lower conductivity protein samples for iso-electric focusing gels. Perfect-FOCUS™ is designed to clean and remove interfering agents from protein samples and concentrate protein solutions that give poor protein spot resolution during 2D electrophoresis.

Numéro de catalogue: 786-124T

UOM: 1 * 1 KIT

Fournisseur: G-Biosciences

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Description: Saponin permeating solution, is used as a cell permeabilising agent.

Numéro de catalogue: J63209.AK

UOM: 1 * 250 mL

Fournisseur: Thermo Fisher Scientific

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Description: For chemiluminecent HRP-based detection of western blotting systems. It is soluble in most polar organic solvents but is insoluble in water.

Numéro de catalogue: J61462.AP

UOM: 1 * 500 mL

Fournisseur: Thermo Fisher Scientific

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Description: These Chemiluminescent Western Blot Kits allow for the detection of primary polyclonal or monoclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane-bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialised formulation of luminol, an extremely sensitive, non radioactive substrate that emits light and allows visualisation using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Because of the extremely high sensitivity of the kit, primary and secondary antibodies can be used at greater dilutions. Primary antibodies currently diluted 1:1000 using an ECL™ substrate is the equivalent of a 1:5000 dilution using the FemtoMax™ substrate.

Numéro de catalogue: ROCKKCA004

UOM: 1 * 1 KIT

Fournisseur: Rockland Immunochemicals

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Description: Le CheLuminate-HRP PicoDetect est un kit complet comprenant des réactifs prêts à l'emploi pour la détection par chimiluminescence de protéines immobilisées (Western blot) ou d'acides nucléiques immobilisés (Southern ou Northern blot), conjugués à de la peroxydase de raifort (HRP) directement ou indirectement.

Numéro de catalogue: A3417.5000

UOM: 1 * 1 KIT

Fournisseur: PanReac AppliChem

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Description: The Fast Western Blot Kits, SuperSignal™ West Dura provide reagents and protocol for reliable and rapid, 1 hour chemiluminescent Western blotting at femtogram levels of sensitivity. These complete Fast Western Blot Kits requires minimal hands on time and yield results comparable to classical Western blotting with SuperSignal™ West Dura Chemiluminescent Substrate. This substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with mouse or rabbit primary antibody.

Numéro de catalogue: PIER35071

UOM: 1 * 1 KIT

Fournisseur: Thermo Fisher Scientific

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