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Kits de marquage des anticorps, colorant Mix-n-Stain™ CF™

Fournisseur: Biotium

92270.EA 125 EUR
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Kits de marquage des anticorps, colorant Mix-n-Stain™ CF™
Systèmes de liaison et de marquage des protéines
Les kits de marquage des anticorps Mix-n-Stain™ CF™ permettent de simplifier la préparation des anticorps marqués par fluorescence.

  • <30 secondes de manipulation plus 30 minutes de temps de marquage
  • Aucune purification nécessaire
  • Peut tolérer les stabilisants des anticorps courants tels que BSA, Tris, azide de sodium et glycérol, en faible quantité
  • Sélection des colorants les plus vifs avec des longueurs d'onde couvrant la totalité des spectres visible et proche IR
  • Permet de marquer n'importe quelle quantité d'anticorps entre 5 g et 100 g
  • Conjugué obtenu stable jusqu'à 6 mois

Mix-n-Stain™ CF® Dye antibody labelling kits allow you to label ≤5 µg up to 100 µg of your antibody in just 30 minutes, with minimal hands-on time and no purification.

Mix-n-Stain™ labeling tolerates common antibody buffer formulations. An ultrafiltration centrifuge vial is provided to quickly remove interfering substances like glycerol, or to concentrate your antibody. With a slight modification in protocol, antibodies can be labelled in the presence of BSA, gelatin, or ascites fluid. Because CF® Dyes are highly water soluble, the presence of other proteins like BSA or gelatin in the labelling reaction has minimal effect on background fluorescence, because any labelled non-antibody proteins readily wash away during immunofluorescence staining.

These kits feature a choice of 32 of Biotium’s next-generation CF® Dyes, which were designed for advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes.

Mix-n-Stain™ antibody labelling kits are very simple to use (see the workflow overview). But before you begin, you must check that your antibody meets the compatibility requirements for labelling, and choose the right labelling protocol. See the product table below for a quick reference of the maximum excitation and emission of each CF® Dye.

Biotium now offer Mix-n-Stain™ Maxi Antibody labelling kits for labelling 1 mg of antibody with CF® Dyes, and Mix-n-Stain™ Nanobody labelling kits. Mix-n-Stain™ kits also are available for labelling antibodies with enzymes (HRP, AP, and GOx), fluorescent proteins (PE, APC, PerCP, and tandem dyes), biotin, or DIG.

Information de livraison: Le kit contient du colorant CF™, un tampon de réaction et un tampon de stockage.
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