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Votre recherche pour: Systèmes de liaison et de marquage des protéines

Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.

Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.


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Biotin kits, Micro HOOK™

Fournisseur: G-Biosciences
Description: The micro HOOK™ Biotin kits are designed to label small amounts of proteins, with each kit designed for 8 to 10 labellings of 50 to 250 µg protein/reaction.

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CF™ Dye lectin conjugates

Fournisseur: Biotium
Description: A lectin labelled with our superior fluorescent CF® dyes is an effective and widely used cell surface stain for yeast, fungi, and mammalian cells.

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Annexin V Near IR CF® dye conjugates

Fournisseur: Biotium
Description: Near-IR Annexin V fluorescent conjugates can be used to detect apoptotic cells and is suitable for <i>in vivo</i> imaging.

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Biotin picolyl azide

Numéro de catalogue: (92186.)
Fournisseur: Biotium
Description: Biotin picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency.
UOM: 1 * 0,5 mg
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Dye tyramide, CF®

Fournisseur: Biotium
Description: Fluorescent CF® dye tyramides are used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolour immunocytochemistry (ICC), immunohistochemistry (IHC), or <i>in situ</i> hybridization (ISH).

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Orange 552 dUTP (lyophilised)

Numéro de catalogue: (ENZOENZ42842L0050)
Fournisseur: ENZO LIFE SCIENCES
Description: Orange 552 dUTP (lyophilised)
UOM: 1 * 50 Nmo

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Orange 552 dUTP

Numéro de catalogue: (ENZOENZ42842)
Fournisseur: ENZO LIFE SCIENCES
Description: Orange 552 dUTP (5-TAMRA-dUTP) can replace TTP in reactions in which it serves as a substrate for <i>E. coli</i> DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Orange 552 dUTP can also be used for multicolor fluorescence labeling.
UOM: 1 * 25 Nmo

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ORGANELLE-ID-RGB® III Assay kit

Numéro de catalogue: (ENZOENZ51032K100)
Fournisseur: ENZO LIFE SCIENCES
Description: TheORGANELLE-ID-RGB® III assay kit contains Golgi apparatus-selective, endoplasmic reticulum-selective and nucleus-selective dyes suitable for live cell staining.
UOM: 1 * 1 KIT

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Lipophilic carbocyanine dyes and CellBrite cytoplasmic membrane labelling kits

Fournisseur: Biotium
Description: Carbocyanine dyes with long hydrocarbon chains have low fluorescence in aqueous phase, but become highly fluorescent upon binding to lipid bilayers, making this class of dyes ideal for staining cell membranes. Carbocyanine dye membrane staining is very stable, with minimal dye transfer between cells. Dilinoleyl carbocynaine dyes have much faster lateral diffusion rates than regular carbocyanine dyes, which facilitates labelling of neurons in tissues.

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CDI amine reactive resin

Numéro de catalogue: (786-404)
Fournisseur: G-Biosciences
Description: CDI amine reactive agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates.
UOM: 1 * 10 mL
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IgG Biotinylation kits, HOOK™

Fournisseur: G-Biosciences
Description: HOOK™ IgG biotinylation kits are designed for the biotinylation of IgG molecules by immobilising the IgG molecules on a solid support. Nickel chelating resin allows for the rapid removal of uncoupled biotin and therefore eliminates the need for further dialysis or desalting of the biotinylated antibody.

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CF® Dye hydrazide

Fournisseur: Biotium
Description: Fluorescent CF® Dye hydrazides are bright, extremely water-soluble, nontoxic molecules, useful as aldehyde-fixable cell tracers. They can also be used to label aldehyde or ketone groups of polysaccharides, carbohydrates or glycoproteins.

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Phalloidin conjugates

Fournisseur: Biotium
Description: Phalloidin is a toxin isolated from the deadly <i>Amanita phalloides</i> mushroom. It is a bicyclic peptide that binds specifically to F-actin. Fluorescent conjugates of phalloidin are convenient tools to investigate the distribution of F-actin in fixed and permeabilised cells or tissue sections. One unit (U) of phallodin is sufficient to stain one sample (200 µl staining volume). A highly selective actin filament stain for fixed and permeabilised cells. Labeled phalloidins have similar affinity for both large and small filaments.

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Conjugués d’annexine V

Fournisseur: Biotium
Description: Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

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Antibody labelling kits, Mix-n-Stain™ Biotin

Fournisseur: Biotium
Description: Mix-n-Stain™ Biotin antibody labelling kits utilise a technology that allows rapid antibody labelling with biotin without a purification step.

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Tyramide amplification kit with HRP goat, anti-rabbit and CF® dye or biotin tyramide

Fournisseur: Biotium
Description: Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.

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