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Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.


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Bicinchoninic acid (BCA) protein assay

Fournisseur: G-Biosciences
Description: The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions.

Certificats

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Micro Bicinchoninic Acid (BCA) protein assay

Fournisseur: G-Biosciences
Description: The Micro Bicinchoninic Acid Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions. This modification of the Bicinchoninic Acid (BCA) Protein Assay is suitable for dilute protein samples (0,5 to 20 µg/ml).

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Compatible Lowry protein assay

Fournisseur: G-Biosciences
Description: These CL (Compatible Lowry) Protein Assay is based on the widely cited protein assay by Lowry et. al. (1951). The CL Protein Assay improves upon the traditional Lowry method to be compatible with common laboratory agents known to interfere with Lowry protein assays such as reducing agents (dithiothreitol (DTT), ß-mercaptoethanol and TCEP), detergents, chelating agents, amines, sugars, strong chaotropic buffers, salts, drugs, antibiotics, cobalt and other common laboratory agents.

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L-Lactate Assay Kit

Numéro de catalogue: (CAYM700510-96)
Fournisseur: Cayman Chemical
Description: A fluorescence-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and saliva.
UOM: 1 * 1 ST
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Dosage de protéines Bradford, qualité protéomique

Numéro de catalogue: (M172-1L)
Fournisseur: VWR Chemicals
Description: Le dosage de protéines Bradford est le dosage colorimétrique privilégié pour quantifier la concentration totale en protéines. Basée sur la formation complexe entre les résidus d'acides aminés basiques et aromatiques avec le colorant Coomassie® bleu brillant G-250, la méthode de Bradford est plus facile, plus rapide et plus sensible que la méthode de Lowry.
UOM: 1 * 1 L

MSMD Certificats


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Protein quantitation assay kits, CB-X™ Protein assay

Fournisseur: G-Biosciences
Description: CB-X™ Protein Assay is a Coomassie dye based protein assay based on the Bradford Protein Assay. For protein samples in simple, uncomplicated aqueous buffers CB-X™ is a highly sensitive, single reagent assay that can be performed in 5 minutes. For complicated protein samples, CB-X™ Protein Assay is supplied with reagents to clean up the samples and remove all reagents and chemicals that interfere with accurate protein estimation in a single step. These reagents include detergents, chaotropes, reducing agents, alkylating agents, sugars, high salt concentrations, buffering agents and chelating agents. The clean up stage and subsequent protein assay is performed in a single tube to ensure no protein loss and to maintain the accuracy of the assay.

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Protein quantitation assay kits, CB™ Protein assay

Fournisseur: G-Biosciences
Description: CB™ Protein Assay, a Coomassie dye based protein assay based on the Bradford Protein Assay, is suitable for the simple and rapid estimation of protein concentration. The binding of protein to the dye results in a colour change from brown to blue. The change in colour density is proportional to protein concentration. Protein estimation can be performed using as little as 0,5 µg protein.

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Osteoclast inhibitory lectin MAB MO XMO

Numéro de catalogue: (USBIO8061-100)
Fournisseur: US Biological
Description: Osteocrin is a recently identified secreted protein expression of which was only detected in bone, peaking just after birth and decreasing markedly with age. A 1280-bp mRNA encodes osteocrin producing a mature protein of 103 amino acids with a molecular mass of 11,4 kD. In primary osteoblastic cell cultures osteocrin expression coincided with matrix formation then decreased in very mature cultures.
UOM: 1 * 1 EA
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PROTEOSTAT® protein refolding and aggregation sensing kit for microplates

Numéro de catalogue: (ENZOENZ51040KP002)
Fournisseur: ENZO LIFE SCIENCES
Description: ProteoStat® Protein refolding and aggregation sensing kit provides a fractional factorial matrix design to facilitate screening of refolding parameters for a specific protein in different buffers, and a simple, homogenous assay format for monitoring protein aggregation using a red-emitting aggregation-sensitive dye. 
UOM: 1 * 1 KIT

New Product


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β-Hydroxybutyrate (Ketone Body) Assay Kit

Numéro de catalogue: (CAYM700190-96)
Fournisseur: Cayman Chemical
Description: A sensitive tool for measuring β-HB levels in plasma, serum, or urine.
UOM: 1 * 1 ST
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PROTEOSTAT® thermal shift stability assay kit

Fournisseur: ENZO LIFE SCIENCES
Description: This kit has been designed for monitoring protein stability under systematic thermal stress conditions.

New Product

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Pierce™ Micro BCA™ Protein Assay Kit and Reagents

Fournisseur: Thermo Fisher Scientific
Description: Micro BCA™ protein assay kit is a specialised version of Pierce™ BCA protein assay for determining the protein concentration of dilute samples.

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Antibody labelling kits, HisTag® Antibody HRP Conjugate kit

Numéro de catalogue: (71840-3)
Fournisseur: Merck Millipore (Novagen)
Description: The HisTag® Antibody HRP Conjugate kit contains a horseradish peroxidase-conjugated HisTag® Monoclonal Antibody that is useful as a single detection reagent, thus eliminating cross-reactivity associated with secondary antibody reagents. It recognises five consecutive histidine residues regardless of the surrounding amino acid context. The purified antibody conjugate produces a strong signal at a 1:1000 to 1:2000 dilution on Western blots and dot blots. The HisTag® Antibody HRP Conjugate also detects the recombinant marker bands in the Novagen Perfect Protein™ Western Markers for convenient visualisation of accurate internal standards on Western blots.
UOM: 1 * 125 µl
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Firefly luciferase flash assay kit, Pierce™

Fournisseur: Thermo Fisher Scientific
Description: The Pierce™ Firefly Luciferase Flash Assay Kit provides reagents to measure red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.

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Compat-Able™ Protein assay preparation reagent set

Numéro de catalogue: (PIER23215)
Fournisseur: Thermo Fisher Scientific
Description: The Compat-Able™ Protein assay preparation reagent set eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.
UOM: 1 * 500 mL
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Coomassie Plus™ protein assay kit, Compat-Able™

Numéro de catalogue: (PIER23239)
Fournisseur: Thermo Fisher Scientific
Description: The Thermo Scientific™ Compat-Able™ Coomassie Plus Protein Assay Kit eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.
UOM: 1 * 1 KIT
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